Laura Bailey


Office: CLB 302



University of Florida
Ph.D. in Analytical Chemistry, 2013-present
Graduate Research Project with Dr. Nick Polfer


Wright State University

B.S. Chemistry; Mathematics minor, April 2013
Undergraduate Research Interests: Gas Phase Energy Transfer Kinetics



Research Intrests:


My research has primarily focused on the fragmentation of metabolites, including small (di- and tri-) peptides, for structural elucidation. Towards this end, I have used collisional induced dissociation (CID), higher-energy collisional dissociation (HCD), ultraviolet photodissociation (UVPD), and infrared multiple photon dissociation (IRMPD) to compare fragmentation patterns.


I have also been involved in the development of a cryogenic linear ion trap (cryoLIT), for the purposes of performing infrared photodissociation (IRPD) spectroscopy. Since these measurements are performed at cryogenic temperatures (20-30 K), a tagging scheme can be employed, which serves to lower the required dissociation energy and simplify the mass spectra. For this project, I was mainly focused on the temperature control and gas handling systems.


Currently, metabolomics suffers from inadequate structural characterization, with approximately 1% of metabolites being fully characterized. While IR spectroscopy is the standard for structural determination in the condensed phase, collection and/or concentration challenges have prohibited the analysis of many metabolites on this platform. Furthermore, large peak widths and limited spectral ranges have limited the usefulness of IR action spectroscopy; however, using the tagging scheme for IRPD spectroscopy allows for single photon dissociation of the tag from an ion, resulting in narrower peak widths (compared to IRMPD) and the collection of the fingerprint IR region from a benchtop IR source. As shown in the example below, metabolites in the same pathway are generally structurally similar, so that, if a library of known structures are compiles, unknown structures and metabolite classes can be determined by the similarity, or dissimilarity, of the IRPD spectra. Therefore, this methodology could be used in the identification of unknown metabolites.



Current projects include investigating the degree of phosphate loss and/or rearrangement in various peptides and differentiating isomeric advanced glycation products using IRPD spectroscopy.




'Making Mass Spectrometry See the Light: The Promises and Challenges of Cryogenic Infrared Ion Spectroscopy as a Bioanalytical Technique', AP Cismesia, LS Bailey, MR Bell, LF Tesler, NC Polfer; J. Am. Soc. Mass Spectrom., 2016


'Infrared ion spectroscopy inside a mass-selective cryogenic 2D linear ion trap', AP Cismesia, LF Tesler, MR Bell, LS Bailey, NC Polfer; J. Mass Spectrom., 2017